Condensation reaction of hexanucleotides containing guanine and cytosine with water soluble carbodiimide
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چکیده
منابع مشابه
Detection of single-base mutations by reaction of DNA heteroduplexes with a water-soluble carbodiimide followed by primer extension: application to products from the polymerase chain reaction
A new method was developed for the detection of single-base mutations in DNA. The polymerase chain reaction was used to prepare DNA fragments of up to 1 kb. Fragments that differed by a single-base were combined, denatured and renatured to generate heteroduplexes. The heteroduplexes were reacted with a water-soluble carbodiimide under conditions in which the carbodiimide modified Gs and Ts that...
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ژورنال
عنوان ژورنال: Nucleic Acids Symposium Series
سال: 2000
ISSN: 0261-3166,1746-8272
DOI: 10.1093/nass/44.1.217